RARS - Antibodies - The Human Protein Atlas

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RARS

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA003979

Antibody HPA004130

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA003979

HPA004130

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA003979, RRID:AB_1856083

Atlas Antibodies Cat#HPA004130, RRID:AB_1856084

VALIDATION SUMMARY

Antibody HPA003979

Antibody HPA004130

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA003979

Antibody HPA004130

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Figure description

Cytoplasmic region of segmented cells in 10x-images

Antibody dilution

1:55

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA004130.

Validated

Antibody staining overlaps with antibody HPA003979.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Antibody dilution

1:38

1:53

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA003979

Antibody HPA004130

Standard validation

Approved

Figure description

Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in Purkinje cells and cells in molecular layer.

Expression

RNA: detected in 37 tissues
Protein: detected in 75 cell types

RNA: detected in 37 tissues
Protein: detected in 46 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:250

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA003979

Antibody HPA004130

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

75.4

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA003979

Antibody HPA004130

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:6000

ANTIGEN INFORMATION

Antibody HPA003979

Antibody HPA004130

Antigen

Recombinant protein fragment

Length (aa)

150

142

Antigen sequence

TIIGESISRLFEFAGYDVLRLNHVGDWGTQFGMLIAHLQDKFPDYLTVSP
PIGDLQVFYKESKKRFDTEEEFKKRAYQCVVLLQGKNPDITKAWKLICDV
SRQELNKIYDALDVSLIERGESFYQDRMNDIVKEFEDRGFVQVDDGRKIV

VVLGEDKKKFKTRSGETVRLMDLLGEGLKRSMDKLKEKERDKVLTAEELN
AAQTSVAYGCIKYADLSHNRLNDYIFSFDKMLDDRGNTAAYLLYAFTRIR
SIARLANIDEEMLQKAARETKILLDHEKEWKLGRCILRFPEI

Matching transcripts

RARS-001 - ENSP00000231572 [100%]

Other gene match

ANTIGEN VIEW

RARS-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.