Cell atlas - RARS - The Human Protein Atlas

GENERAL INFORMATION
Gene name	RARS
Gene description	Arginyl-tRNA synthetase
Protein class	Disease related genes Enzymes Plasma proteins Potential drug targets Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	2
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the cytosol. In addition localized to the nucleoplasm.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Cytosol (validated)
Additional location	In addition localized to the Nucleoplasm (approved)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA003979 , HPA004130
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the cytosol. In addition localized to the nucleoplasm.

Main location

Cytosol (validated)

Additional location

Nucleoplasm (approved)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA003979	A-431	Nucleoplasm
HPA003979	A-431	Cytosol
HPA003979	U-2 OS	Nucleoplasm
HPA003979	U-2 OS	Cytosol
HPA003979	U-251 MG	Nucleoplasm
HPA003979	U-251 MG	Cytosol
HPA004130	A-431	Cytosol
HPA004130	U-2 OS	Cytosol
HPA004130	U-251 MG	Nucleoplasm
HPA004130	U-251 MG	Cytosol

siRNA VALIDATION

Antibody

siRNA validation

Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

Cytoplasmic region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

28%

44%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Sample	Cell line
HPA004130	siRNA 1	U-2 OS
HPA004130	siRNA 2	U-2 OS
HPA004130	scrambled	U-2 OS

GENE INFORMATION
Gene name	RARS (HGNC Symbol)
Synonyms	DALRD1
Description	Arginyl-tRNA synthetase (HGNC Symbol)
Entrez gene summary	Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Jul 2008]
Chromosome	5
Cytoband	q34
Chromosome location (bp)	168486445 - 168519299
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000113643 (version 83.38)
Entrez gene	5917
UniProt	P54136 (UniProt - Evidence at protein level)
neXtProt	NX_P54136
Antibodypedia	RARS antibodies

PROTEIN BROWSER

RARS-001	RARS-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
RARS-001 ENSP00000231572 ENST00000231572	P54136 [Direct mapping] Arginine--tRNA ligase, cytoplasmic Show all	Enzymes ENZYME proteins Ligase SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Plasma proteins Disease related genes Potential drug targets Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000049 [tRNA binding] GO:0000166 [nucleotide binding] GO:0004812 [aminoacyl-tRNA ligase activity] GO:0004814 [arginine-tRNA ligase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005739 [mitochondrion] GO:0005829 [cytosol] GO:0006418 [tRNA aminoacylation for protein translation] GO:0006420 [arginyl-tRNA aminoacylation] GO:0010467 [gene expression] GO:0016020 [membrane] GO:0017101 [aminoacyl-tRNA synthetase multienzyme complex] GO:0034618 [arginine binding] GO:0070062 [extracellular exosome] Show all	660 aa 75.4 kDa	No	0
RARS-201 ENSP00000486284 ENST00000626454	E5RI24 [Direct mapping] Arginine--tRNA ligase, cytoplasmic Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0004814 [arginine-tRNA ligase activity] Show all	58 aa 6.2 kDa	No	0