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Antibody staining overlaps with antibody HPA069808.
Validated
Antibody staining overlaps with antibody HPA069752.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm.
Antibody dilution
1:40
1:51
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA064854
Antibody HPA069752
Antibody HPA069808
Standard validation
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 65 cell types
Retrieval
HIER pH6
Antibody dilution
1:675
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA064854
Antibody HPA069752
Antibody HPA069808
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
123.9, 121.7
123.9, 121.7
123.9
Antibody dilution
1:170
1:130
1:170
PROTEIN ARRAY
Antibody HPA064854
Antibody HPA069752
Antibody HPA069808
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
