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BCL6

ANTIBODY INFORMATION
 
 

Antibody HPA004899

  

Antibody HPA050645

  

Antibody CAB000307

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
  

Product name

 HPA004899   HPA050645   M7211  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

      

Released in version

 3   16   1  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA004899, RRID:AB_1078273   Atlas Antibodies Cat#HPA050645, RRID:AB_2681199   Dako Cat#M7211, RRID:AB_2063451  

VALIDATION SUMMARY
 
 

Antibody HPA004899

  

Antibody HPA050645

  

Antibody CAB000307

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

Supported     Supported  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004899

  

Antibody HPA050645

  

Antibody CAB000307

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & the Golgi apparatus.    

Antibody dilution

 1:95   1:200    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004899

  

Antibody HPA050645

  

Antibody CAB000307

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.     Immunohistochemical staining of human lymph node shows moderate nuclear positivity in reaction center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 34 cell types		     RNA: detected in 37 tissues
Protein: detected in 16 cell types		  

Retrieval

 HIER pH6     HIER pH9  

Antibody dilution

 1:150     1:10  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004899

  

Antibody HPA050645

  

Antibody CAB000307

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 78.8, 72.4   78.8, 72.4   78.8, 72.4, 13.9, 11.8  

Antibody dilution

 1:250   1:1060   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004899

  

Antibody HPA050645

  

Antibody CAB000307

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:8450    

ANTIGEN INFORMATION
 
 

Antibody HPA004899

  

Antibody HPA050645

  

Antibody CAB000307

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 129   103    

Antigen sequence

 
NIYSPKETIPEEARSDMHYSVAEGLKPAAPSARNAPYFPCDKASKEEERP
SSEDEIALHFEPPNAPLNRKGLVSPQSPQKSDCQPNSPTESCSSKNACIL
QASGSPPAKSPTDPKACNWKKYKFIVLNS
  
EHVVDTCRKFIKASEAEMVSAIKPPREEFLNSRMLMPQDIMAYRGREVVE
NNLPLRSAPGCESRAFAPSLYSGLSTPPASYSMYSHLPVSSLLFSDEEFR
DVR
  

											 
																				

																		

										
											
Matching transcripts

												
																					BCL6-001 - ENSP00000384371 [100%] 
BCL6-004 - ENSP00000232014 [100%] 
BCL6-010 - ENSP00000413122 [100%] 
BCL6-201 - ENSP00000479784 [100%] 

											 
																						BCL6-001 - ENSP00000384371 [100%] 
BCL6-004 - ENSP00000232014 [100%] 
BCL6-010 - ENSP00000413122 [100%] 
BCL6-201 - ENSP00000479784 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
BCL6-001
BCL6-004
BCL6-010
BCL6-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA004899

												
										 
																				
											
											
											
Antibody HPA050645

												
										 
																				
											
											
											
Antibody CAB000307

												
										 
																		

																	

										
											
1.

												
																						Molecular analysis of precursor lesions in familial pancreatic cancer

	Crnogorac-Jurcevic T et al

	PLoS One 2013;8(1):e54830

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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