We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Antibody dilution
1:196
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA055839
Antibody CAB004526
Standard validation
Supported
Figure description
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Expression
RNA: detected in 37 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055839
Antibody CAB004526
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
93
93
Antibody dilution
1:650
1:500
PROTEIN ARRAY
Antibody HPA055839
Antibody CAB004526
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:7800
ANTIGEN INFORMATION
Antibody HPA055839
Antibody CAB004526
Antigen
Recombinant protein fragment
Recombinant protein
Length (aa)
51
Antigen sequence
KFSHLPAKERQTIVELAKMFLNRINYWHLEAPSQRRLRSPNDDISGYKEN
Y
