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ROPN1B

ANTIBODY INFORMATION
 
 

Antibody HPA052530

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA052530  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 ROPN1 - ENSG00000065371 [96%]
  

Released in version

 11  

References

  

Proper citation

 Atlas Antibodies Cat#HPA052530, RRID:AB_2681861  

VALIDATION SUMMARY
 
 

Antibody HPA052530

  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA052530

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 9 tissues
Protein: detected in 48 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:500  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA052530

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 24, 23.9, 14.8, 13.2, 11.7, 8.8  

Antibody dilution

 1:270  

PROTEIN ARRAY
 
 

Antibody HPA052530

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2150  

ANTIGEN INFORMATION
 
 

Antibody HPA052530

  

Antigen

 Recombinant protein fragment  

Length (aa)

 45  

Antigen sequence

 
QDLIQWGADYFEALSRGETPPVRERSERVALCNWAELTPELLKIL
 

Matching transcripts

 ROPN1B-001 - ENSP00000251776 [100%]
ROPN1B-003 - ENSP00000426271 [100%]
ROPN1B-007 - ENSP00000425548 [100%]
ROPN1B-010 - ENSP00000423058 [100%]
 

Other gene match

 ROPN1 - ENSG00000065371 [96%]
  
ANTIGEN VIEW
ROPN1B-001
ROPN1B-003
ROPN1B-007
ROPN1B-010
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.