Fields »
ABOUT
HELP
BLOG
ROPN1

ANTIBODY INFORMATION
 
 

Antibody HPA052530

  

Antibody CAB014875

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA052530   2105  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

 ROPN1B - ENSG00000114547 [100%]
    

Released in version

 11   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA052530, RRID:AB_2681861    

VALIDATION SUMMARY
 
 

Antibody HPA052530

  

Antibody CAB014875

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA052530

  

Antibody CAB014875

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong positivity in spermatids.  

Expression

 RNA: detected in 4 tissues
Protein: detected in 48 cell types		   RNA: detected in 4 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:20  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA052530

  

Antibody CAB014875

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 24, 23.9, 14.8, 13.2, 11.7, 8.8   23.9, 14.8, 13.2, 8.8, 6.7  

Antibody dilution

 1:270   1:500  

PROTEIN ARRAY
 
 

Antibody HPA052530

  

Antibody CAB014875

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2150    

ANTIGEN INFORMATION
 
 

Antibody HPA052530

  

Antibody CAB014875

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 45    

Antigen sequence

 
QDLIQWGADYFEALSRGETPPVRERSERVALCNWAELTPELLKIL
   

Matching transcripts

 ROPN1-001 - ENSP00000184183 [96%]
ROPN1-005 - ENSP00000385919 [96%]
ROPN1-008 - ENSP00000418931 [96%]
ROPN1-009 - ENSP00000420310 [96%]
ROPN1-013 - ENSP00000417067 [96%]
ROPN1-201 - ENSP00000483603 [96%]
   

Other gene match

 ROPN1B - ENSG00000114547 [100%]
    
ANTIGEN VIEW
ROPN1-001
ROPN1-005
ROPN1-008
ROPN1-009
ROPN1-013
ROPN1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.