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ANTIBODY INFORMATION
 
 

Antibody HPA005550

  

Antibody CAB016721

  

Antibody CAB016792

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   BioPorto Diagnostics A/S
   BioPorto Diagnostics A/S
  

Product name

 HPA005550   ABS 001-07   HYB 237-01  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

      

Released in version

 3   4   4  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA005550, RRID:AB_1079687      

VALIDATION SUMMARY
 
 

Antibody HPA005550

  

Antibody CAB016721

  

Antibody CAB016792

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005550

  

Antibody CAB016721

  

Antibody CAB016792

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human fallopian tube shows positivity in plasma.   Immunohistochemical staining of human fallopian tube shows positivity plasma.   Immunohistochemical staining of human colon shows positivity in plasma.  

Expression

 RNA: detected in 4 tissues
Protein: detected in 32 cell types		   RNA: detected in 4 tissues
Protein: detected in 54 cell types		   RNA: detected in 4 tissues
Protein: detected in 58 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:15   1:15  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA005550

  

Antibody CAB016721

  

Antibody CAB016792

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 55.4, 52.1, 26.3   55.4, 52.1, 26.3, 19.9, 14.1, 13   55.4, 52.1, 26.3, 19.9, 14.1, 13  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA005550

  

Antibody CAB016721

  

Antibody CAB016792

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:1000      

ANTIGEN INFORMATION
 
 

Antibody HPA005550

  

Antibody CAB016721

  

Antibody CAB016792

  

Antigen

 Recombinant protein fragment   Recombinant protein   Native protein  

Length (aa)

 139      

Antigen sequence

 
AHCMDESKKLLVRLGEYDLRRWEKWELDLDIKEVFVHPNYSKSTTDNDIA
LLHLAQPATLSQTIVPICLPDSGLAERELNQAGQETLVTGWGYHSSREKE
AKRNRTFVLNFIKIPVVPHNECSEVMSNMVSENMLCAGI
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					PROC-001 - ENSP00000234071 [100%] 
PROC-002 - ENSP00000386679 [100%] 
PROC-012 - ENSP00000384225 [90%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PROC-001
PROC-002
PROC-012
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA005550

												
										 
																				
											
											
											
Antibody CAB016721

												
										 
																				
											
											
											
Antibody CAB016792

												
										 
																		

																	

										
											
1.

												
																						Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas

	Wu CC et al

	Mol Cell Proteomics 2010;9(6):1100-17

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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