Fields »
ABOUT
HELP
BLOG
UAP1

ANTIBODY INFORMATION
 
 

Antibody HPA014659

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA014659  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

 1  

Proper citation

 Atlas Antibodies Cat#HPA014659, RRID:AB_1857409  

VALIDATION SUMMARY
 
 

Antibody HPA014659

  
Immunocytochemistry

Supported  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA014659

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.  

Antibody dilution

 1:18  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014659

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 47 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:25  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA014659

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 58.8, 58.7, 57  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA014659

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA014659

  

Antigen

 Recombinant protein fragment  

Length (aa)

 145  

Antigen sequence

 
NDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAI
EGFNQSSHQKNVDARMEPVPREVLGSATRDQDQLQAWESEGLFQISQNKV
AVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQAERILKLQQ
 

Matching transcripts

 UAP1-001 - ENSP00000356903 [100%]
UAP1-002 - ENSP00000356902 [100%]
UAP1-003 - ENSP00000356901 [100%]
UAP1-201 - ENSP00000271469 [100%]
 

Other gene match

  
ANTIGEN VIEW
UAP1-001
UAP1-002
UAP1-003
UAP1-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA014659

  

1.

 N-linked glycosylation supports cross-talk between receptor tyrosine kinases and androgen receptor
Itkonen HM et al
PLoS One 2013;8(5):e65016
Application: WB 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.