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RPA2

ANTIBODY INFORMATION
 
 

Antibody HPA026306

  

Antibody HPA026309

  

Antibody CAB016538

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA026306   HPA026309   sc-53496  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 6   6   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA026306, RRID:AB_10598913   Atlas Antibodies Cat#HPA026309, RRID:AB_10601572   Santa Cruz Biotechnology Cat#sc-53496, RRID:AB_670974  

VALIDATION SUMMARY
 
 

Antibody HPA026306

  

Antibody HPA026309

  

Antibody CAB016538

  
Immunocytochemistry


Validated

Validated
  

Validated
   Supported  
Immunohistochemistry


Validated
   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA026306

  

Antibody HPA026309

  

Antibody CAB016538

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		      

     

Figure description

 Nuclear region of segmented cells in 10x-images      

Antibody dilution

 1:141      
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA026309.		  
Validated

Antibody staining overlaps with antibody HPA026306.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.  

Antibody dilution

 1:100   1:10   1:19  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026306

  

Antibody HPA026309

  

Antibody CAB016538

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

     

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.   Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 64 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 79 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:25   1:75  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026306

  

Antibody HPA026309

  

Antibody CAB016538

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 38.8, 30.2, 29.2, 13.2   38.8, 30.2, 29.2, 19.4   38.8, 30.2, 29.2, 19.4, 13.2  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA026306

  

Antibody HPA026309

  

Antibody CAB016538

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA026306

  

Antibody HPA026309

  

Antibody CAB016538

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 98   75    

Antigen sequence

 
HMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNL
IKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDH
  
WNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTI
SQLLSATLVDEVFRIGNVEISQVTI
  

											 
																				

																		

										
											
Matching transcripts

												
																					RPA2-001 - ENSP00000363021 [100%] 
RPA2-002 - ENSP00000363017 [100%] 
RPA2-004 - ENSP00000363015 [100%] 
RPA2-005 - ENSP00000413541 [100%] 

											 
																						RPA2-001 - ENSP00000363021 [100%] 
RPA2-002 - ENSP00000363017 [96%] 
RPA2-004 - ENSP00000363015 [96%] 
RPA2-003 - ENSP00000387649 [95%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
RPA2-001
RPA2-002
RPA2-003
RPA2-004
RPA2-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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