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RPA2

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GENERAL INFORMATION

Gene name

 RPA2

Gene description

 Replication protein A2, 32kDa

Protein class

 Cancer-related genes
Plasma proteins
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 5
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Mainly localized to the nucleoplasm. In addition localized to the nuclear bodies.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nucleoplasm (validated)

Additional location

 In addition localized to the Nuclear bodies (validated)

Cell cycle dependency

 Cell cycle dependent expression determined by custom assay

DATA RELIABILITY

Reliability score

 Validated based on 3 antibodies.
CAB016538 , HPA026306 , HPA026309
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ASSAYS
RNA expression
Human cells
Custom data
siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Mainly localized to the nucleoplasm. In addition localized to the nuclear bodies.

Main location

 Nucleoplasm (validated)

Additional location

 Nuclear bodies (validated)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
CAB016538 A-431 Nucleoplasm
CAB016538 U-2 OS Nucleoplasm
Nuclear bodies
CAB016538 U-251 MG Nucleoplasm
Nuclear bodies
HPA026306 A-431 Nucleoplasm
HPA026306 U-2 OS Nucleoplasm
Nuclear bodies
HPA026306 U-251 MG Nucleoplasm
HPA026309 A-431 Nucleoplasm
HPA026309 U-2 OS Nucleoplasm
Nuclear bodies
HPA026309 U-251 MG Nucleoplasm

CUSTOM DATA

 

 

 
Toggle channels

Thumbnail

Antibody

Cell line

Assay

Description
HPA026306 U-2 OS Image Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. RPA2 is mostly expressed during G1.

CELL CYCLE

Summary

 Expression has cell cycle dependency according to custom assay

siRNA VALIDATION

Antibody

 HPA026306

siRNA validation

 Validated: Significant downregulation > 25% by one siRNA.

Analysis performed in

 Nuclear region of segmented cells in 10x-images

siRNA 2

RFI

 51%

p-value

 < 0.01
 

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

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Thumbnail 40x

Antibody

Sample

Cell line
HPA026306 siRNA 2 U-2 OS
HPA026306 scrambled U-2 OS

GENE INFORMATION

Gene name

 RPA2

Synonyms

Description

 Replication protein A2, 32kDa (HGNC Symbol)

Entrez gene summary

Chromosome

 1

Cytoband

 p35.3

Chromosome location (bp)

 27891524 - 27914746

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000117748 (version 83.38)

Entrez gene

 6118

UniProt

 P15927 (UniProt - Evidence at protein level)

neXtProt

 NX_P15927

Antibodypedia

 RPA2 antibodies

PROTEIN BROWSER
RPA2-001
RPA2-002
RPA2-003
RPA2-004
RPA2-005

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
RPA2-001
ENSP00000363021
ENST00000373912
P15927 [Direct mapping]
Replication protein A 32 kDa subunit
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000722 [telomere maintenance via recombination]
GO:0000723 [telomere maintenance]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0000784 [nuclear chromosome, telomeric region]
GO:0000785 [chromatin]
GO:0003676 [nucleic acid binding]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0003697 [single-stranded DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005662 [DNA replication factor A complex]
GO:0006260 [DNA replication]
GO:0006271 [DNA strand elongation involved in DNA replication]
GO:0006281 [DNA repair]
GO:0006283 [transcription-coupled nucleotide-excision repair]
GO:0006284 [base-excision repair]
GO:0006289 [nucleotide-excision repair]
GO:0006297 [nucleotide-excision repair, DNA gap filling]
GO:0006298 [mismatch repair]
GO:0006302 [double-strand break repair]
GO:0006310 [DNA recombination]
GO:0010569 [regulation of double-strand break repair via homologous recombination]
GO:0016605 [PML body]
GO:0019899 [enzyme binding]
GO:0019903 [protein phosphatase binding]
GO:0019985 [translesion synthesis]
GO:0031571 [mitotic G1 DNA damage checkpoint]
GO:0031625 [ubiquitin protein ligase binding]
GO:0032201 [telomere maintenance via semi-conservative replication]
GO:0033683 [nucleotide-excision repair, DNA incision]
GO:0034605 [cellular response to heat]
GO:0035861 [site of double-strand break]
GO:0036297 [interstrand cross-link repair]
GO:0042276 [error-prone translesion synthesis]
GO:0042769 [DNA damage response, detection of DNA damage]
GO:0070911 [global genome nucleotide-excision repair]
GO:0070987 [error-free translesion synthesis]
GO:1900034 [regulation of cellular response to heat]
GO:2000001 [regulation of DNA damage checkpoint]
Show all
 270 aa
29.2 kDa 		No 0
RPA2-002
ENSP00000363017
ENST00000373909
P15927 [Direct mapping]
Replication protein A 32 kDa subunit
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000722 [telomere maintenance via recombination]
GO:0000723 [telomere maintenance]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0000784 [nuclear chromosome, telomeric region]
GO:0000785 [chromatin]
GO:0003676 [nucleic acid binding]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0003697 [single-stranded DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005662 [DNA replication factor A complex]
GO:0006260 [DNA replication]
GO:0006271 [DNA strand elongation involved in DNA replication]
GO:0006281 [DNA repair]
GO:0006283 [transcription-coupled nucleotide-excision repair]
GO:0006284 [base-excision repair]
GO:0006289 [nucleotide-excision repair]
GO:0006297 [nucleotide-excision repair, DNA gap filling]
GO:0006298 [mismatch repair]
GO:0006302 [double-strand break repair]
GO:0006310 [DNA recombination]
GO:0010569 [regulation of double-strand break repair via homologous recombination]
GO:0016605 [PML body]
GO:0019899 [enzyme binding]
GO:0019903 [protein phosphatase binding]
GO:0019985 [translesion synthesis]
GO:0031571 [mitotic G1 DNA damage checkpoint]
GO:0031625 [ubiquitin protein ligase binding]
GO:0032201 [telomere maintenance via semi-conservative replication]
GO:0033683 [nucleotide-excision repair, DNA incision]
GO:0034605 [cellular response to heat]
GO:0035861 [site of double-strand break]
GO:0036297 [interstrand cross-link repair]
GO:0042276 [error-prone translesion synthesis]
GO:0042769 [DNA damage response, detection of DNA damage]
GO:0047485 [protein N-terminus binding]
GO:0070911 [global genome nucleotide-excision repair]
GO:0070987 [error-free translesion synthesis]
GO:1900034 [regulation of cellular response to heat]
GO:2000001 [regulation of DNA damage checkpoint]
Show all
 278 aa
30.2 kDa 		No 0
RPA2-003
ENSP00000387649
ENST00000444045
Q5TEJ7 [Direct mapping]
Replication protein A 32 kDa subunit
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003676 [nucleic acid binding]
GO:0005654 [nucleoplasm]
Show all
 179 aa
19.4 kDa 		No 0
RPA2-004
ENSP00000363015
ENST00000313433
P15927 [Direct mapping]
Replication protein A 32 kDa subunit
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000722 [telomere maintenance via recombination]
GO:0000723 [telomere maintenance]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0000784 [nuclear chromosome, telomeric region]
GO:0000785 [chromatin]
GO:0003676 [nucleic acid binding]
GO:0003684 [damaged DNA binding]
GO:0003697 [single-stranded DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005662 [DNA replication factor A complex]
GO:0006260 [DNA replication]
GO:0006271 [DNA strand elongation involved in DNA replication]
GO:0006281 [DNA repair]
GO:0006283 [transcription-coupled nucleotide-excision repair]
GO:0006284 [base-excision repair]
GO:0006289 [nucleotide-excision repair]
GO:0006297 [nucleotide-excision repair, DNA gap filling]
GO:0006298 [mismatch repair]
GO:0006302 [double-strand break repair]
GO:0010569 [regulation of double-strand break repair via homologous recombination]
GO:0016605 [PML body]
GO:0019899 [enzyme binding]
GO:0019903 [protein phosphatase binding]
GO:0019985 [translesion synthesis]
GO:0031571 [mitotic G1 DNA damage checkpoint]
GO:0031625 [ubiquitin protein ligase binding]
GO:0032201 [telomere maintenance via semi-conservative replication]
GO:0033683 [nucleotide-excision repair, DNA incision]
GO:0034605 [cellular response to heat]
GO:0035861 [site of double-strand break]
GO:0036297 [interstrand cross-link repair]
GO:0042276 [error-prone translesion synthesis]
GO:0042769 [DNA damage response, detection of DNA damage]
GO:0070911 [global genome nucleotide-excision repair]
GO:0070987 [error-free translesion synthesis]
GO:1900034 [regulation of cellular response to heat]
GO:2000001 [regulation of DNA damage checkpoint]
Show all
 358 aa
38.8 kDa 		No 0
RPA2-005
ENSP00000413541
ENST00000419958
Q5TEJ0 [Direct mapping]
Replication protein A 32 kDa subunit
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005654 [nucleoplasm]
Show all
 122 aa
13.2 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.