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ANTIBODY INFORMATION
 
 

Antibody HPA042550

  

Antibody HPA059859

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042550   HPA059859  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA042550, RRID:AB_2678048   Atlas Antibodies Cat#HPA059859, RRID:AB_2684145  

VALIDATION SUMMARY
 
 

Antibody HPA042550

  

Antibody HPA059859

  
Immunocytochemistry

Uncertain    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042550

  

Antibody HPA059859

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & plasma membrane.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042550

  

Antibody HPA059859

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA059859 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA042550 across 46 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human esophagus shows moderate membranous positivity in superficial layers of squamous epithelial cells.   Immunohistochemical staining of human nasopharynx shows cytoplasamic positivity in respiratory epithelial cells.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 31 cell types		   RNA: detected in 34 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:30000   1:750  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042550

  

Antibody HPA059859

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 204.7, 204.5   204.7, 204.5  

Antibody dilution

 1:250   1:140  

PROTEIN ARRAY
 
 

Antibody HPA042550

  

Antibody HPA059859

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:1700  

ANTIGEN INFORMATION
 
 

Antibody HPA042550

  

Antibody HPA059859

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 95   97  

Antigen sequence

 
LLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNEL
LRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLL
  
AKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEES
HLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELLL
 

Matching transcripts

 PPL-001 - ENSP00000340510 [100%]
PPL-003 - ENSP00000465640 [100%]
  PPL-001 - ENSP00000340510 [100%]
PPL-003 - ENSP00000465640 [100%]
 

Other gene match

    
ANTIGEN VIEW
PPL-001
PPL-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.