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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:20
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA019678
Antibody CAB037139
Antibody CAB037208
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and distinct cytoplasmic and nuclear positivity in Leydig cells.
Expression
RNA: detected in 37 tissues Protein: detected in 25 cell types
RNA: detected in 37 tissues Protein: detected in 49 cell types
RNA: detected in 37 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:225
1:3000
1:4000
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019678
Antibody CAB037139
Antibody CAB037208
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.2
26.2, 25.5, 21.9
26.2, 25.5, 21.9
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA019678
Antibody CAB037139
Antibody CAB037208
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.