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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli fibrillar center.
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & nuclear bodies.
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies.
Antibody dilution
1:119
1:52
1:13
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027885
Antibody HPA068537
Antibody CAB017524
Antibody CAB069400
Standard validation
Supported
Figure description
Immunohistochemical staining of human testis shows nuclear positivity in testis.
Expression
RNA: detected in 37 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
Antibody dilution
1:10000
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027885
Antibody HPA068537
Antibody CAB017524
Antibody CAB069400
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
62.6
62.6
62.6, 4.1
62.6, 4.1
Antibody dilution
1:250
1:170
1:500
1:500
PROTEIN ARRAY
Antibody HPA027885
Antibody HPA068537
Antibody CAB017524
Antibody CAB069400
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
