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ANTIBODY INFORMATION
 
 

Antibody HPA001031

  

Antibody CAB000469

  

Antibody CAB047315

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   AbD Serotec
   NCI-CPTC
  

Product name

 HPA001031   MCA2069T   CPTC-CSTA-1  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

      

Released in version

 2   1   9  

References

 2      

Proper citation

 Atlas Antibodies Cat#HPA001031, RRID:AB_1078610      

VALIDATION SUMMARY
 
 

Antibody HPA001031

  

Antibody CAB000469

  

Antibody CAB047315

  
Immunocytochemistry

Uncertain      
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Approved      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001031

  

Antibody CAB000469

  

Antibody CAB047315

  

Standard validation

 Uncertain      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.      

Antibody dilution

 1:35      

Literature conformity

 The subcellular location is not consistent with literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001031

  

Antibody CAB000469

  

Antibody CAB047315

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human skin shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human esophagus shows strong positivity in squamous epithelial cells.   Immunohistochemical staining of human oral mucosa shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 30 cell types		   RNA: detected in 34 tissues
Protein: detected in 21 cell types		   RNA: detected in 34 tissues
Protein: detected in 17 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:50   1:20000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001031

  

Antibody CAB000469

  

Antibody CAB047315

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 11   11, 7.1   11, 7.1  

Antibody dilution

 1:500   1:700   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001031

  

Antibody CAB000469

  

Antibody CAB047315

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA001031

  

Antibody CAB000469

  

Antibody CAB047315

  

Antigen

 Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 80      

Antigen sequence

 
IPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGT
NYYIKVRAGDNKYMHLKVFKSLPGQNEDLV
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CSTA-001 - ENSP00000264474 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CSTA-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001031

												
										 
																				
											
											
											
Antibody CAB000469

												
										 
																				
											
											
											
Antibody CAB047315

												
										 
																		

																	

										
											
1.

												
																						Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma

	Asplund A et al

	Br J Dermatol 2008;158(3):527-38

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies

	Ek S et al

	Mol Cell Proteomics 2006;5(6):1072-81

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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