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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus & nucleoli and GFP expression in nucleus & nucleoli.
Antibody dilution
1:8
Standard validation
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Antibody dilution
1:8
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA041838
Antibody HPA048257
Antibody CAB070171
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemical staining of human kidney shows strong nuclear and cytoplasmic positivity in renal tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 62 cell types
RNA: detected in 37 tissues Protein: detected in 80 cell types
RNA: detected in 37 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:35
1:750
1:1500
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041838
Antibody HPA048257
Antibody CAB070171
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
50.8, 48.9, 47
50.8, 48.9, 47
50.8, 48.9, 47, 25.7, 18.7
Antibody dilution
1:250
1:470
1:500
PROTEIN ARRAY
Antibody HPA041838
Antibody HPA048257
Antibody CAB070171
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
