RIOK1 - Antibodies - The Human Protein Atlas

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RIOK1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA017866

Antibody HPA051446

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA017866

HPA051446

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA017866, RRID:AB_2669670

Atlas Antibodies Cat#HPA051446, RRID:AB_2681488

VALIDATION SUMMARY

Antibody HPA017866

Antibody HPA051446

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA017866

Antibody HPA051446

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli, nuclear speckles & cytosol.

Antibody dilution

1:70

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA017866

Antibody HPA051446

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA051446 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA017866 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.

Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 46 cell types

RNA: detected in 37 tissues
Protein: detected in 42 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:300

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA017866

Antibody HPA051446

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

65.6

Antibody dilution

1:250

1:350

PROTEIN ARRAY

Antibody HPA017866

Antibody HPA051446

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:2800

ANTIGEN INFORMATION

Antibody HPA017866

Antibody HPA051446

Antigen

Recombinant protein fragment

Length (aa)

144

Antigen sequence

NVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKE
ERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQD
NILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSEC

DWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFEN
KINLDKLNVTDSVINKVTEKSRQKEADMYRIKD

Matching transcripts

RIOK1-001 - ENSP00000369162 [100%]

Other gene match

ANTIGEN VIEW

RIOK1-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.