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STK35

ANTIBODY INFORMATION
 
 

Antibody HPA026452

  

Antibody HPA051673

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA026452   HPA051673  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA026452, RRID:AB_2671861   Atlas Antibodies Cat#HPA051673, RRID:AB_2681569  

VALIDATION SUMMARY
 
 

Antibody HPA026452

  

Antibody HPA051673

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA026452

  

Antibody HPA051673

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line HaCaT shows localization to nucleus & nuclear bodies.    

Antibody dilution

 1:7    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026452

  

Antibody HPA051673

  

Standard validation

   Uncertain  

   

Figure description

   Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 52 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:350  

Literature conformity

   Not consistent with gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026452

  

Antibody HPA051673

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 58.1   58.1  

Antibody dilution

 1:250   1:200  

PROTEIN ARRAY
 
 

Antibody HPA026452

  

Antibody HPA051673

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:5600  

ANTIGEN INFORMATION
 
 

Antibody HPA026452

  

Antibody HPA051673

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 62   43  

Antigen sequence

 
TSLKRRHQNVVQFEECVLQRNGLAQRMSHGNKSSQLYLRLVETSLKGERI
LGYAEEPCYLWF
  
TSLKRRHQNVVQFEECVLQRNGLAQRMSHGNKSSQLYLRLVET
 

Matching transcripts

 STK35-001 - ENSP00000370891 [100%]
  STK35-001 - ENSP00000370891 [100%]
 

Other gene match

    
ANTIGEN VIEW
STK35-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.