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Immunohistochemical staining of human lactating breast shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human lactating breast shows strong membranous positivity in glandular cells.
Expression
RNA: detected in 3 tissues Protein: detected in 0 cell types
RNA: detected in 3 tissues Protein: detected in 21 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:2000
1:6500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA035659
Antibody HPA057031
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
21.7, 20.8, 20.7, 19.9
21.7, 20.8, 20.7, 19.9, 9
Antibody dilution
1:250
1:820
PROTEIN ARRAY
Antibody HPA035659
Antibody HPA057031
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
