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Cytoplasmic region of segmented cells in 10x-images
Antibody dilution
1:76
Formal validation: Independent
Validated
Antibody staining overlaps with antibody HPA044227.
Validated
Antibody staining overlaps with antibody HPA041082.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
Antibody dilution
1:57
1:76
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA041082
Antibody HPA044227
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA044227 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA041082 across 46 cell lines.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules. Moderate staining was observed in cells in glomeruli.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules. Moderate staining was observed in cells in glomeruli.
Expression
RNA: detected in 37 tissues Protein: detected in 78 cell types
RNA: detected in 37 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:120
1:450
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041082
Antibody HPA044227
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
80.1, 74.3, 57.2
80.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA041082
Antibody HPA044227
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
