HSPA2 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

HSPA2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA000798

Antibody HPA052504

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA000798

HPA052504

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

HSPA8 - ENSG00000109971 [95%]
HSPA6 - ENSG00000173110 [95%]
HSPA1B - ENSG00000204388 [100%]
HSPA1A - ENSG00000204389 [100%]
HSPA1L - ENSG00000204390 [95%]

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA000798, RRID:AB_1079090

Atlas Antibodies Cat#HPA052504, RRID:AB_2681854

VALIDATION SUMMARY

Antibody HPA000798

Antibody HPA052504

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA000798

Antibody HPA052504

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.

Antibody dilution

1:96

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA000798

Antibody HPA052504

Standard validation

Approved

Figure description

Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in decidual cells.

Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.

Expression

RNA: detected in 35 tissues
Protein: detected in 70 cell types

RNA: detected in 35 tissues
Protein: detected in 29 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:1000

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

Standard validation

Approved

Figure description

Labelling of many neurons throughout the brain. Immunoreactivity in soma and nuclei.

WESTERN BLOT

Antibody HPA000798

Antibody HPA052504

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

71, 70.9, 70.4, 70.1, 70, 68.8, 55, 53.5, 51.9, 45.3, 34.8

Antibody dilution

1:500

1:480

PROTEIN ARRAY

Antibody HPA000798

Antibody HPA052504

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1000

1:3850

ANTIGEN INFORMATION

Antibody HPA000798

Antibody HPA052504

Antigen

Recombinant protein fragment

Length (aa)

150

Antigen sequence

ILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRD
RVAAKNALESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRN
QMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGSGGGGSGASGGPTIEE