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HSPA6

ANTIBODY INFORMATION
 
 

Antibody HPA028549

  

Antibody HPA052504

  

Antibody CAB017452

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Enzo Life Sciences
  

Product name

 HPA028549   HPA052504   ADI-SPA-756  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

   HSPA8 - ENSG00000109971 [95%]
HSPA2 - ENSG00000126803 [95%]
HSPA1B - ENSG00000204388 [100%]
HSPA1A - ENSG00000204389 [100%]
HSPA1L - ENSG00000204390 [95%]
    

Released in version

 6   12   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA028549, RRID:AB_10601817   Atlas Antibodies Cat#HPA052504, RRID:AB_2681854   Enzo Life Sciences Cat#ADI-SPA-756, RRID:AB_10618032  

VALIDATION SUMMARY
 
 

Antibody HPA028549

  

Antibody HPA052504

  

Antibody CAB017452

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028549

  

Antibody HPA052504

  

Antibody CAB017452

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.    

Antibody dilution

   1:96    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028549

  

Antibody HPA052504

  

Antibody CAB017452

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in the cells of seminiferous tubules.   Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.   Immunohistochemical staining of human thyroid gland shows strong cytoplasmic and nuclear positivity in glandular cells.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 55 cell types		   RNA: detected in 31 tissues
Protein: detected in 29 cell types		   RNA: detected in 31 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:750   1:1000   1:80  

Literature conformity

 Consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028549

  

Antibody HPA052504

  

Antibody CAB017452

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 71   71, 70.9, 70.4, 70.1, 70, 68.8, 55, 53.5, 51.9, 45.3, 34.8   71  

Antibody dilution

 1:250   1:480   1:500  

PROTEIN ARRAY
 
 

Antibody HPA028549

  

Antibody HPA052504

  

Antibody CAB017452

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:30000   1:3850    

ANTIGEN INFORMATION
 
 

Antibody HPA028549

  

Antibody HPA052504

  

Antibody CAB017452

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 99   22    

Antigen sequence

 
EVERMVHEAEQYKAEDEAQRDRVAAKNSLEAHVFHVKGSLQEESLRDKIP
EEDRRKMQDKCREVLAWLEHNQLAEKEEYEHQKRELEQICRPIFSRLYG
  
ACERAKRTLSSSTQASLEIDSL
   

Matching transcripts

 HSPA6-001 - ENSP00000310219 [100%]
  HSPA6-001 - ENSP00000310219 [95%]
   

Other gene match

   HSPA8 - ENSG00000109971 [95%]
HSPA2 - ENSG00000126803 [95%]
HSPA1B - ENSG00000204388 [100%]
HSPA1A - ENSG00000204389 [100%]
HSPA1L - ENSG00000204390 [95%]
    
ANTIGEN VIEW
HSPA6-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.