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ANTIBODY INFORMATION
 
 

Antibody HPA002110

  

Antibody HPA045507

  

Antibody CAB016169

  

Antibody CAB062558

  

Antibody CAB068219

  

Antibody CAB068220

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002110   HPA045507   sc-23903   AMAb90667   AMAb90643   AMAb90644  

Host species

 Rabbit   Rabbit   Mouse   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G   Protein A/G   Protein A/G  

Other gene match

            

Released in version

 4   10   4   12   13   13  

References

 8            

Proper citation

 Atlas Antibodies Cat#HPA002110, RRID:AB_1855512   Atlas Antibodies Cat#HPA045507, RRID:AB_2679356   Santa Cruz Biotechnology Cat#sc-23903, RRID:AB_628165   Atlas Antibodies Cat#AMAb90667, RRID:AB_2665627   Atlas Antibodies Cat#AMAb90643, RRID:AB_2665618   Atlas Antibodies Cat#AMAb90644, RRID:AB_2665619  

VALIDATION SUMMARY
 
 

Antibody HPA002110

  

Antibody HPA045507

  

Antibody CAB016169

  

Antibody CAB062558

  

Antibody CAB068219

  

Antibody CAB068220

  
Immunocytochemistry

Supported            
Immunohistochemistry


Validated
   Supported   Supported   Supported  

Validated
   Supported  
Western blot

Supported   Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported          

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002110

  

Antibody HPA045507

  

Antibody CAB016169

  

Antibody CAB062558

  

Antibody CAB068219

  

Antibody CAB068220

  

Standard validation

 Supported            

           

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, microtubule organizing center & vesicles.            

Antibody dilution

 1:88            

Literature conformity

 The subcellular location is supported by literature.            

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002110

  

Antibody HPA045507

  

Antibody CAB016169

  

Antibody CAB062558

  

Antibody CAB068219

  

Antibody CAB068220

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		        
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

       
   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 40 cell lines.         Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported   Supported   Supported   Supported   Supported  

           

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.   Immunohistochemical staining of human kidney shows moderate membranous positivity in renal glomeruli.   Immunohistochemical staining of human kidney shows cytoplasmic positivity in glomeruli.   Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in glomeruli.   Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in glomeruli.   Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 34 cell types		   RNA: detected in 35 tissues
Protein: detected in 8 cell types		   RNA: detected in 35 tissues
Protein: detected in 14 cell types		   RNA: detected in 35 tissues
Protein: detected in 6 cell types		   RNA: detected in 35 tissues
Protein: detected in 8 cell types		   RNA: detected in 35 tissues
Protein: detected in 12 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:125   1:300   1:250   1:2000   1:5000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002110

  

Antibody HPA045507

  

Antibody CAB016169

  

Antibody CAB062558

  

Antibody CAB068219

  

Antibody CAB068220

  

Standard validation

 Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

           

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401657)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 58.6, 55.4   58.6, 55.4   58.6, 55.4   58.6, 55.4   58.6, 55.4   58.6, 55.4  

Antibody dilution

 1:250   1:250   1:500   1:500   1:1000   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA002110

  

Antibody HPA045507

  

Antibody CAB016169

  

Antibody CAB062558

  

Antibody CAB068219

  

Antibody CAB068220

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		          

           

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.          

Antibody dilution

 1:3000   1:500          

ANTIGEN INFORMATION
 
 

Antibody HPA002110

  

Antibody HPA045507

  

Antibody CAB016169

  

Antibody CAB062558

  

Antibody CAB068219

  

Antibody CAB068220

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

 138   142          

Antigen sequence

 
LPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVL
NLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVV
VKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGD
  
QNATQTTTDSSNKTAPTPASSVTIMATDTAQQSTVPTSKANEILASVKAT
TLGVSSDSPGTTTLAQQVSGPVNTTVARGGGSGNPTTTIESPKSTKSADT
TTVATSTATAKPNTTSSQNGAEDTTNSGGKSSHSVTTDLTST
  

											 
																						

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					PODXL-001 - ENSP00000319782 [100%] 
PODXL-005 - ENSP00000367817 [100%] 

											 
																						PODXL-001 - ENSP00000319782 [100%] 
PODXL-005 - ENSP00000367817 [100%] 

											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PODXL-001
PODXL-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002110

												
										 
																				
											
											
											
Antibody HPA045507

												
										 
																				
											
											
											
Antibody CAB016169

												
										 
																				
											
											
											
Antibody CAB062558

												
										 
																				
											
											
											
Antibody CAB068219

												
										 
																				
											
											
											
Antibody CAB068220

												
										 
																		

																	

										
											
1.

												
																						Methylation of an intronic region regulates miR-199a in testicular tumor malignancy

	Cheung HH et al

	Oncogene 2011;30(31):3404-15

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Overexpression of podocalyxin-like protein is an independent factor of poor prognosis in colorectal cancer

	Larsson A et al

	Br J Cancer 2011;105(5):666-72

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						Sialofucosylated podocalyxin is a functional E- and L-selectin ligand expressed by metastatic pancreatic cancer cells

	Dallas MR et al

	Am J Physiol Cell Physiol 2012;303(6):C616-24

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Membranous expression of podocalyxin-like protein is an independent factor of poor prognosis in urothelial bladder cancer

	Boman K et al

	Br J Cancer 2013;108(11):2321-8

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
5.

												
																						Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays

	Forsström B et al

	Mol Cell Proteomics 2014;13(6):1585-97

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
6.

												
																						Prognostic and predictive significance of podocalyxin-like protein expression in pancreatic and periampullary adenocarcinoma

	Heby M et al

	BMC Clin Pathol 2015;15:10

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
7.

												
																						Podocalyxin Is a Marker of Poor Prognosis in Pancreatic Ductal Adenocarcinoma

	Saukkonen K et al

	PLoS One 2015;10(6):e0129012

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
8.

												
																						Expression of podocalyxin-like protein is an independent prognostic biomarker in resected esophageal and gastric adenocarcinoma

	Borg D et al

	BMC Clin Pathol 2016;16:13

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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by the Knut & Alice Wallenberg foundation.