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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PODXL
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

PODXL

Gene description

Podocalyxin-like

Protein class

Plasma proteins
Predicted membrane proteins

Predicted localization

Membrane

Number of transcripts

2
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (NTERA-2, TIME)
HPA (normal tissue):Mixed

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Distinct expression in glomerular podocytes, endothelial cells, glandular cells in fallopian tube, uterus and seminal vesicle.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Supported based on 6 antibodies.
HPA002110 , HPA045507 , CAB016169 , CAB062558 , CAB068219 , CAB068220
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (favourable).
Renal cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Endometrial cancer

STAINING SUMMARY

HPA002110
HPA045507
CAB016169
CAB062558
CAB068219
CAB068220

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong cytoplasmic positivity was observed in several gliomas, endometrial, testicular and ovarian cancers along with few colorectal and pancreatic cancers. Remaining cancer tissues were weakly stained or negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Malignant tissues were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong membranous and cytoplasmic immunoreactivity was observed in most testicular cancer as well as cases of endometrial cancers, squamous cell carcinomas and high grade gliomas. Malignant cells in general were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Strong cytoplasmic and luminal membranous positivity was observed in fraction of cells in endometrial and testicular cancers. A few gliomas and pancreatic cancers exhibited moderate to strong cytoplasmic immunoreactivity. Adenocarcinoma of head & neck cancer and a few ovarian cancers showed strong luminal membranous staining. Remaining cancer tissues were mainly negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong luminal membranous staining was observed in several ovarian, endometrial and a few testicular cancers. Remaining cancer tissues were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cases of endometrial and ovarian cancers, several cases of embryonal carcinomas of testis, duct carcinomas of breast and pancreatic cancers along with rare cases of liver cancers exhibited moderate to strong membranous immunoreactivity. Remaining cancer tissues were negative.

GENE INFORMATION

Gene name

PODXL (HGNC Symbol)

Synonyms

Gp200, PC, PCLP

Description

Podocalyxin-like (HGNC Symbol)

Entrez gene summary

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

Chromosome

7

Cytoband

q32.3

Chromosome location (bp)

131500262 - 131558217

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000128567 (version 83.38)

Entrez gene

5420

UniProt

O00592 (UniProt - Evidence at protein level)

neXtProt

NX_O00592

Antibodypedia

PODXL antibodies

PROTEIN BROWSER

PODXL-001
PODXL-005

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

PODXL-001
ENSP00000319782
ENST00000322985
O00592 [Direct mapping]
Podocalyxin
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001726 [ruffle]
GO:0005515 [protein binding]
GO:0005615 [extracellular space]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0005887 [integral component of plasma membrane]
GO:0007155 [cell adhesion]
GO:0007162 [negative regulation of cell adhesion]
GO:0016324 [apical plasma membrane]
GO:0016477 [cell migration]
GO:0022407 [regulation of cell-cell adhesion]
GO:0022408 [negative regulation of cell-cell adhesion]
GO:0030027 [lamellipodium]
GO:0030175 [filopodium]
GO:0030335 [positive regulation of cell migration]
GO:0031528 [microvillus membrane]
GO:0032534 [regulation of microvillus assembly]
GO:0033634 [positive regulation of cell-cell adhesion mediated by integrin]
GO:0036057 [slit diaphragm]
GO:0045121 [membrane raft]
GO:0070062 [extracellular exosome]
GO:0072015 [glomerular visceral epithelial cell development]
GO:0072175 [epithelial tube formation]
Show all
526 aa
55.4 kDa
Yes 1
PODXL-005
ENSP00000367817
ENST00000378555
O00592 [Direct mapping]
Podocalyxin
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001726 [ruffle]
GO:0005515 [protein binding]
GO:0005615 [extracellular space]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0005887 [integral component of plasma membrane]
GO:0007155 [cell adhesion]
GO:0007162 [negative regulation of cell adhesion]
GO:0016324 [apical plasma membrane]
GO:0016477 [cell migration]
GO:0022407 [regulation of cell-cell adhesion]
GO:0022408 [negative regulation of cell-cell adhesion]
GO:0030027 [lamellipodium]
GO:0030175 [filopodium]
GO:0030335 [positive regulation of cell migration]
GO:0031528 [microvillus membrane]
GO:0032534 [regulation of microvillus assembly]
GO:0033634 [positive regulation of cell-cell adhesion mediated by integrin]
GO:0036057 [slit diaphragm]
GO:0044297 [cell body]
GO:0045121 [membrane raft]
GO:0050900 [leukocyte migration]
GO:0070062 [extracellular exosome]
GO:0072015 [glomerular visceral epithelial cell development]
GO:0072175 [epithelial tube formation]
Show all
558 aa
58.6 kDa
Yes 1

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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.