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Immunohistochemical staining of human retina shows strong positivity in rod outer segments.
Immunohistochemical staining of human retina shows strong positivity in rod outer segments.
Expression
RNA: detected in 33 tissues Protein: detected in 4 cell types
RNA: detected in 33 tissues Protein: detected in 0 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:250
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA013562
Antibody HPA014083
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
39.1
39.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA013562
Antibody HPA014083
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
