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PVRL2

ANTIBODY INFORMATION
 
 

Antibody HPA012759

  

Antibody HPA073459

  

Antibody CAB026138

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA012759   HPA073459   AF2229  

Host species

 Rabbit   Rabbit   Goat  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 4   16   6  

References

      

Proper citation

 Atlas Antibodies Cat#HPA012759, RRID:AB_1846227      

VALIDATION SUMMARY
 
 

Antibody HPA012759

  

Antibody HPA073459

  

Antibody CAB026138

  
Immunocytochemistry

  Uncertain    
Immunohistochemistry


Validated
    

Validated
  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA012759

  

Antibody HPA073459

  

Antibody CAB026138

  

Standard validation

   Uncertain    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center & cell junctions.    

Antibody dilution

   1:23    

Literature conformity

   The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012759

  

Antibody HPA073459

  

Antibody CAB026138

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human testis shows cytoplasmic and membranous positivity in cells in seminiferus ducts.     Immunohistochemical staining of human testis shows distinct membranous positivity in cells of seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 54 cell types		     RNA: detected in 37 tissues
Protein: detected in 31 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:100     1:40  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012759

  

Antibody HPA073459

  

Antibody CAB026138

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 57.7, 51.4   57.7   57.7, 51.4, 22.6, 6.1  

Antibody dilution

 1:250   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA012759

  

Antibody HPA073459

  

Antibody CAB026138

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:600    

ANTIGEN INFORMATION
 
 

Antibody HPA012759

  

Antibody HPA073459

  

Antibody CAB026138

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 124   79    

Antigen sequence

 
VRVQVLPEVRGQLGGTVELPCHLLPPVPGLYISLVTWQRPDAPANHQNVA
AFHPKMGPSFPSPKPGSERLSFVSAKQSTGQDTEAELQDATLALHGLTVE
DEGNYTCEFATFPKGSVRGMTWLR
  
EEDEDLEGPPSYKPPTPKAKLEAQEMPSQLFTLGASEHSPLKTPYFDAGA
SCTEQEMPRYHELPTLEERSGPLHPGATS
  

											 
																				

																		

										
											
Matching transcripts

												
																					PVRL2-001 - ENSP00000252485 [100%] 
PVRL2-002 - ENSP00000252483 [100%] 

											 
																						PVRL2-002 - ENSP00000252483 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PVRL2-001
PVRL2-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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