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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus.
Antibody dilution
1:60
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA017060
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomerular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
Antibody dilution
1:500
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017060
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401380)
Target mass (kDa)
19.8
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA017060
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
