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Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
Antibody dilution
1:4
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA064047
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in renal tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 51 cell types
Retrieval
HIER pH6
Antibody dilution
1:80
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA064047
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
36.7, 26.7
Antibody dilution
1:80
PROTEIN ARRAY
Antibody HPA064047
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
