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ANTIBODY INFORMATION
 
 

Antibody HPA022434

  

Antibody HPA022953

  

Antibody HPA022959

  

Antibody HPA028758

  

Antibody CAB007783

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Epitomics
  

Product name

 HPA022434   HPA022953   HPA022959   HPA028758   1699-1  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

          

Released in version

 5   5   5   6   3  

References

          

Proper citation

 Atlas Antibodies Cat#HPA022434, RRID:AB_1844513   Atlas Antibodies Cat#HPA022953, RRID:AB_1844514   Atlas Antibodies Cat#HPA022959, RRID:AB_1844515   Atlas Antibodies Cat#HPA028758, RRID:AB_10603575    

VALIDATION SUMMARY
 
 

Antibody HPA022434

  

Antibody HPA022953

  

Antibody HPA022959

  

Antibody HPA028758

  

Antibody CAB007783

  
Immunocytochemistry

Supported   Supported        
Immunohistochemistry


Validated
   Approved  

Validated
   Approved   Approved  
Western blot

Supported   Supported   Supported   Supported   Supported  
Protein array

Supported   Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA022434

  

Antibody HPA022953

  

Antibody HPA022959

  

Antibody HPA028758

  

Antibody CAB007783

  

Standard validation

 Supported   Supported        

         

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.        

Antibody dilution

 1:10   1:10        

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA022434

  

Antibody HPA022953

  

Antibody HPA022959

  

Antibody HPA028758

  

Antibody CAB007783

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		      

   
     

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA022959 across 74 cell types.		     Distribution of protein expression (antibody staining).
Spearman correlation with HPA022434 across 74 cell types.		      
 

Standard validation

 Approved   Approved   Approved   Approved   Approved  

         

Figure description

 Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic and nuclear positivity in islet cells.   Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human kidney shows distinct nuclear and cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 66 cell types		   RNA: detected in 37 tissues
Protein: detected in 45 cell types		   RNA: detected in 37 tissues
Protein: detected in 52 cell types		   RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:35   1:90   1:20   1:200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA022434

  

Antibody HPA022953

  

Antibody HPA022959

  

Antibody HPA028758

  

Antibody CAB007783

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

         

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 120.8, 119.8   120.8, 119.8, 91.1   120.8, 119.8   120.8, 119.8, 91.1, 12.3   120.8, 119.8, 91.1, 14.2, 12.3  

Antibody dilution

 1:250   1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA022434

  

Antibody HPA022953

  

Antibody HPA022959

  

Antibody HPA028758

  

Antibody CAB007783

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:500   1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA022434

  

Antibody HPA022953

  

Antibody HPA022959

  

Antibody HPA028758

  

Antibody CAB007783

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 97   98   101   90    

Antigen sequence

 
AEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDI
KKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGV
  
CANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPA
QEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITE
  
YICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIW
TMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSL
M
  
TTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQNLVVKPDQLIKRRGKLG
LVGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPF
  

											 
																				

																		

										
											
Matching transcripts

												
																					ACLY-001 - ENSP00000253792 [100%] 
ACLY-002 - ENSP00000377474 [100%] 
ACLY-003 - ENSP00000466259 [100%] 
ACLY-201 - ENSP00000345398 [100%] 

											 
																						ACLY-001 - ENSP00000253792 [100%] 
ACLY-002 - ENSP00000377474 [100%] 
ACLY-003 - ENSP00000466259 [100%] 
ACLY-004 - ENSP00000445349 [100%] 
ACLY-201 - ENSP00000345398 [100%] 

											 
																						ACLY-001 - ENSP00000253792 [100%] 
ACLY-002 - ENSP00000377474 [100%] 
ACLY-003 - ENSP00000466259 [100%] 
ACLY-201 - ENSP00000345398 [100%] 

											 
																						ACLY-001 - ENSP00000253792 [100%] 
ACLY-002 - ENSP00000377474 [100%] 
ACLY-003 - ENSP00000466259 [100%] 
ACLY-201 - ENSP00000345398 [100%] 
ACLY-005 - ENSP00000468705 [99%] 
ACLY-004 - ENSP00000445349 [82%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ACLY-001
ACLY-002
ACLY-003
ACLY-004
ACLY-005
ACLY-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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