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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

ACLY

Gene description

ATP citrate lyase

Protein class

Cancer-related genes
Citric acid cycle related proteins
Enzymes
Plasma proteins
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

7
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and nuclear expression in several tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data.

Reliability score

Approved based on 5 antibodies.
HPA022434 , HPA022953 , HPA022959 , HPA028758 , CAB007783
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in cervical cancer (unfavourable) and liver cancer (unfavourable).
Cervical cancer p<0.001
Liver cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Renal cancer

STAINING SUMMARY

HPA022434
HPA022953
HPA022959
HPA028758
CAB007783

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate cytoplasmic staining, often combined with nuclear positivity, was observed in most cancer tissues. Several cases of renal cancer and rostate cancer were strongly stained.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues displayed weak to moderate cytoplasmic immunoreactivity in most cases. Carcinoids displayed moderate to strong positivity. Most squamous cell carcinoma, basal cells carcinomas and several gliomas and lymphomas were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Several cases of prostate, renal, endometrial cancers, lymphoma and melanoma displayed moderate to strong cytoplasmic positivity. Remaining cancer tissues were weakly stained or negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Weak to moderate cytoplasmic staining often combined with nuclear positivity was observed in most cancer tissues. Gliomas, skin, testicular, lung, gastric and pancreatic cancers were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues displayed moderate to strong cytoplasmic and nuclear immunoreactivity.

GENE INFORMATION

Gene name

ACLY (HGNC Symbol)

Synonyms

ACL, ATPCL, CLATP

Description

ATP citrate lyase (HGNC Symbol)

Entrez gene summary

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

Chromosome

17

Cytoband

q21.2

Chromosome location (bp)

41866908 - 41930542

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000131473 (version 83.38)

Entrez gene

47

UniProt

P53396 (UniProt - Evidence at protein level)

neXtProt

NX_P53396

Antibodypedia

ACLY antibodies

PROTEIN BROWSER

ACLY-001
ACLY-002
ACLY-003
ACLY-004
ACLY-005
ACLY-006
ACLY-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ACLY-001
ENSP00000253792
ENST00000352035
P53396 [Direct mapping]
ATP-citrate synthase
A0A024R1T9 [Target identity:100%; Query identity:100%]
ATP citrate lyase, isoform CRA_b
Show all
Enzymes
   ENZYME proteins
   Transferases
   MEMSAT3 predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Citric acid cycle related proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003824 [catalytic activity]
GO:0003878 [ATP citrate synthase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005739 [mitochondrion]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006084 [acetyl-CoA metabolic process]
GO:0006085 [acetyl-CoA biosynthetic process]
GO:0006101 [citrate metabolic process]
GO:0006112 [energy reserve metabolic process]
GO:0006629 [lipid metabolic process]
GO:0006633 [fatty acid biosynthetic process]
GO:0008152 [metabolic process]
GO:0008610 [lipid biosynthetic process]
GO:0009346 [citrate lyase complex]
GO:0015936 [coenzyme A metabolic process]
GO:0016020 [membrane]
GO:0019432 [triglyceride biosynthetic process]
GO:0031325 [positive regulation of cellular metabolic process]
GO:0035338 [long-chain fatty-acyl-CoA biosynthetic process]
GO:0044255 [cellular lipid metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0046872 [metal ion binding]
GO:0046912 [transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer]
GO:0048037 [cofactor binding]
GO:0070062 [extracellular exosome]
Show all
1101 aa
120.8 kDa
No 0
ACLY-002
ENSP00000377474
ENST00000393896
P53396 [Direct mapping]
ATP-citrate synthase
A0A024R1Y2 [Target identity:100%; Query identity:100%]
ATP citrate lyase, isoform CRA_a
Show all
Enzymes
   ENZYME proteins
   Transferases
   MEMSAT3 predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Citric acid cycle related proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003824 [catalytic activity]
GO:0003878 [ATP citrate synthase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006085 [acetyl-CoA biosynthetic process]
GO:0006101 [citrate metabolic process]
GO:0006112 [energy reserve metabolic process]
GO:0008152 [metabolic process]
GO:0008610 [lipid biosynthetic process]
GO:0009346 [citrate lyase complex]
GO:0015936 [coenzyme A metabolic process]
GO:0016020 [membrane]
GO:0019432 [triglyceride biosynthetic process]
GO:0031325 [positive regulation of cellular metabolic process]
GO:0035338 [long-chain fatty-acyl-CoA biosynthetic process]
GO:0044255 [cellular lipid metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0046872 [metal ion binding]
GO:0046912 [transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer]
GO:0048037 [cofactor binding]
GO:0070062 [extracellular exosome]
Show all
1091 aa
119.8 kDa
No 0
ACLY-003
ENSP00000466259
ENST00000590151
P53396 [Direct mapping]
ATP-citrate synthase
A0A024R1T9 [Target identity:100%; Query identity:100%]
ATP citrate lyase, isoform CRA_b
Show all
Enzymes
   ENZYME proteins
   Transferases
   MEMSAT3 predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Citric acid cycle related proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003824 [catalytic activity]
GO:0003878 [ATP citrate synthase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006085 [acetyl-CoA biosynthetic process]
GO:0006101 [citrate metabolic process]
GO:0006112 [energy reserve metabolic process]
GO:0008152 [metabolic process]
GO:0008610 [lipid biosynthetic process]
GO:0009346 [citrate lyase complex]
GO:0015936 [coenzyme A metabolic process]
GO:0016020 [membrane]
GO:0019432 [triglyceride biosynthetic process]
GO:0031325 [positive regulation of cellular metabolic process]
GO:0035338 [long-chain fatty-acyl-CoA biosynthetic process]
GO:0044255 [cellular lipid metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0046872 [metal ion binding]
GO:0046912 [transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer]
GO:0048037 [cofactor binding]
GO:0070062 [extracellular exosome]
Show all
1101 aa
120.8 kDa
No 0
ACLY-004
ENSP00000445349
ENST00000537919
P53396 [Direct mapping]
ATP-citrate synthase
Show all
Enzymes
   ENZYME proteins
   Transferases
Predicted intracellular proteins
Plasma proteins
Citric acid cycle related proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003824 [catalytic activity]
GO:0003878 [ATP citrate synthase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006085 [acetyl-CoA biosynthetic process]
GO:0006112 [energy reserve metabolic process]
GO:0008152 [metabolic process]
GO:0008610 [lipid biosynthetic process]
GO:0009346 [citrate lyase complex]
GO:0015936 [coenzyme A metabolic process]
GO:0016020 [membrane]
GO:0019432 [triglyceride biosynthetic process]
GO:0031325 [positive regulation of cellular metabolic process]
GO:0035338 [long-chain fatty-acyl-CoA biosynthetic process]
GO:0044255 [cellular lipid metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0046872 [metal ion binding]
GO:0046912 [transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer]
GO:0048037 [cofactor binding]
GO:0070062 [extracellular exosome]
Show all
830 aa
91.1 kDa
No 0
ACLY-005
ENSP00000468705
ENST00000592970
K7ESG8 [Direct mapping]
ATP-citrate synthase
Show all
Predicted intracellular proteins
Citric acid cycle related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
Show all
109 aa
12.3 kDa
No 0
ACLY-006
ENSP00000464717
ENST00000590770
K7EIE7 [Direct mapping]
ATP-citrate synthase
Show all
Predicted intracellular proteins
Citric acid cycle related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
133 aa
14.2 kDa
No 0
ACLY-201
ENSP00000345398
ENST00000353196
P53396 [Direct mapping]
ATP-citrate synthase
A0A024R1Y2 [Target identity:100%; Query identity:100%]
ATP citrate lyase, isoform CRA_a
Show all
Enzymes
   ENZYME proteins
   Transferases
   MEMSAT3 predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Citric acid cycle related proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003824 [catalytic activity]
GO:0003878 [ATP citrate synthase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006085 [acetyl-CoA biosynthetic process]
GO:0006101 [citrate metabolic process]
GO:0006112 [energy reserve metabolic process]
GO:0008152 [metabolic process]
GO:0008610 [lipid biosynthetic process]
GO:0009346 [citrate lyase complex]
GO:0015936 [coenzyme A metabolic process]
GO:0016020 [membrane]
GO:0019432 [triglyceride biosynthetic process]
GO:0031325 [positive regulation of cellular metabolic process]
GO:0035338 [long-chain fatty-acyl-CoA biosynthetic process]
GO:0044255 [cellular lipid metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0046872 [metal ion binding]
GO:0046912 [transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer]
GO:0048037 [cofactor binding]
GO:0070062 [extracellular exosome]
Show all
1091 aa
119.8 kDa
No 0

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