RAP1GAP2 - Antibodies - The Human Protein Atlas

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RAP1GAP2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA022148

Antibody HPA022896

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA022148

HPA022896

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA022148, RRID:AB_1849474

Atlas Antibodies Cat#HPA022896, RRID:AB_1849476

VALIDATION SUMMARY

Antibody HPA022148

Antibody HPA022896

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA022148

Antibody HPA022896

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.

Antibody dilution

1:20

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA022148

Antibody HPA022896

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal and glial cells.

Expression

RNA: detected in 35 tissues
Protein: detected in 73 cell types

RNA: detected in 35 tissues
Protein: detected in 69 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:250

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA022148

Antibody HPA022896

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

80.1, 78.4, 77.9

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA022148

Antibody HPA022896

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA022148

Antibody HPA022896

Antigen

Recombinant protein fragment

Length (aa)

105

140

Antigen sequence

MFGRKRSVSFGGFGWIDKTMLASLKVKKQELANSSDATLPDRPLSPPLTA
PPTMKSSEFFEMLEKMQGIKLEEQKPGPQKNKDDYIPYPSIDEVVEKGGP
YPQVI

EDPENVGTPTSLGSSICEEEEEDNLSPNTFGYKLECKGEARAYRRHFLGK
DHLNFYCTGSSLGNLILSVKCEEAEGIEYLRVILRSKLKTVHERIPLAGL
SKLPSVPQIAKAFCDDAVGLRFNPVLYPKASQMIVSYDEH

Matching transcripts

RAP1GAP2-001 - ENSP00000254695 [100%]
RAP1GAP2-201 - ENSP00000444890 [100%]
RAP1GAP2-002 - ENSP00000389824 [86%]
RAP1GAP2-003 - ENSP00000439688 [82%]

RAP1GAP2-001 - ENSP00000254695 [100%]
RAP1GAP2-002 - ENSP00000389824 [100%]
RAP1GAP2-003 - ENSP00000439688 [100%]
RAP1GAP2-201 - ENSP00000444890 [100%]

Other gene match

ANTIGEN VIEW

RAP1GAP2-001	RAP1GAP2-002	RAP1GAP2-003	RAP1GAP2-201

RELEVANT PUBLICATIONS

Antibody HPA022148

Antibody HPA022896

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods
Jakobsen L et al
EMBO J 2011;30(8):1520-35

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.