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ANTIBODY INFORMATION
 
 

Antibody HPA027367

  

Antibody HPA027396

  

Antibody CAB005036

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Epitomics
  

Product name

 HPA027367   HPA027396   1568-1  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

      

Released in version

 5   5   2  

References

   1    

Proper citation

 Atlas Antibodies Cat#HPA027367, RRID:AB_1847216   Atlas Antibodies Cat#HPA027396, RRID:AB_1847217    

VALIDATION SUMMARY
 
 

Antibody HPA027367

  

Antibody HPA027396

  

Antibody CAB005036

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Approved   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027367

  

Antibody HPA027396

  

Antibody CAB005036

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human blood vessel shows distinct positivity of plasma.  

Expression

 RNA: detected in 4 tissues
Protein: detected in 37 cell types		   RNA: detected in 4 tissues
Protein: detected in 58 cell types		   RNA: detected in 4 tissues
Protein: detected in 15 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:35   1:50   1:200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA027367

  

Antibody HPA027396

  

Antibody CAB005036

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 25   25   25, 11.6, 10.4, 5.4  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA027367

  

Antibody HPA027396

  

Antibody CAB005036

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA027367

  

Antibody HPA027396

  

Antibody CAB005036

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 77   71    

Antigen sequence

 
MSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFS
YATKRQDNEILIFWSKDIGYSFTVGGS
  
VRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVNMWDFVL
SPDEINTIYLGGPFSPNVLNW
  

											 
																				

																		

										
											
Matching transcripts

												
																					CRP-001 - ENSP00000255030 [100%] 

											 
																						CRP-001 - ENSP00000255030 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CRP-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA027367

												
										 
																				
											
											
											
Antibody HPA027396

												
										 
																				
											
											
											
Antibody CAB005036

												
										 
																		

																	

										
											
1.

												
																					
											 
																							Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria

	Bachmann J et al

	PLoS Pathog 2014;10(4):e1004038

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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