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EPHB2

ANTIBODY INFORMATION
 
 

Antibody HPA071200

  

Antibody CAB013647

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA071200   1989.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 16   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA071200, RRID:AB_2686360    

VALIDATION SUMMARY
 
 

Antibody HPA071200

  

Antibody CAB013647

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Approved  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA071200

  

Antibody CAB013647

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.    

Antibody dilution

 1:8    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA071200

  

Antibody CAB013647

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in cells in seminiferus ducts.  

Expression

   RNA: detected in 29 tissues
Protein: detected in 80 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:350  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA071200

  

Antibody CAB013647

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 117.5, 110, 109.9, 105.6, 53.3   117.5, 110, 109.9, 105.6, 53.3  

Antibody dilution

 1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA071200

  

Antibody CAB013647

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA071200

  

Antibody CAB013647

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 64    

Antigen sequence

 
CRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLD
MPCTTIPSAPQAVI
  

											 
																				

																		

										
											
Matching transcripts

												
																					EPHB2-001 - ENSP00000383053 [100%] 
EPHB2-002 - ENSP00000363763 [100%] 
EPHB2-003 - ENSP00000363761 [100%] 
EPHB2-004 - ENSP00000363758 [100%] 
EPHB2-201 - ENSP00000444174 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
EPHB2-001
EPHB2-002
EPHB2-003
EPHB2-004
EPHB2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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