LARS - Antibodies - The Human Protein Atlas

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LARS

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA036424

Antibody HPA040881

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036424

HPA040881

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA036424, RRID:AB_10670102

Atlas Antibodies Cat#HPA040881, RRID:AB_2677185

VALIDATION SUMMARY

Antibody HPA036424

Antibody HPA040881

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA036424

Antibody HPA040881

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA040881.

Validated

Antibody staining overlaps with antibody HPA036424.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & cytosol.

Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies & cytosol.

Antibody dilution

1:26

1:7

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA036424

Antibody HPA040881

Standard validation

Supported

Figure description

Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 51 cell types

RNA: detected in 37 tissues
Protein: detected in 59 cell types

Retrieval

HIER pH6

Antibody dilution

1:25

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036424

Antibody HPA040881

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

134.5, 128

134.5, 18.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA036424

Antibody HPA040881

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA036424

Antibody HPA040881

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

EHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRE
RNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVL

KWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHTFSLSKCEF
AVGYQRLKGKCCLFPFGLHCTGMPIKACADKLK

Matching transcripts

LARS-001 - ENSP00000377954 [100%]
LARS-002 - ENSP00000426005 [100%]

LARS-001 - ENSP00000377954 [100%]
LARS-202 - ENSP00000481381 [100%]

Other gene match

ANTIGEN VIEW

LARS-001	LARS-002	LARS-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.