CA1 - Antibodies - The Human Protein Atlas

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CA1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA006558

Antibody CAB025790

Provider

Atlas Antibodies
Sigma-Aldrich

R&D Systems

Product name

HPA006558

MAB2180

Host species

Rabbit

Rat

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA006558, RRID:AB_1845989

VALIDATION SUMMARY

Antibody HPA006558

Antibody CAB025790

Immunohistochemistry

Western blot

Uncertain

Protein array

Approved

IMMUNOHISTOCHEMISTRY

Antibody HPA006558

Antibody CAB025790

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in most cells of red and white pulp.

Expression

RNA: detected in 12 tissues
Protein: detected in 14 cell types

RNA: detected in 12 tissues
Protein: detected in 15 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

1:10000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA006558

Antibody CAB025790

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.

Figure description

Target mass (kDa)

28.9, 27.8, 19.3, 16.4, 16.3

28.9, 27.8, 21.4, 19.7, 19.3, 16.4, 16.3, 15.1, 14, 13.3, 11.2, 9.4, 2.6

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA006558

Antibody CAB025790

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA006558

Antibody CAB025790

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

139

Antigen sequence

NGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEII
NVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVD
GVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMK

Matching transcripts

CA1-001 - ENSP00000430656 [100%]
CA1-003 - ENSP00000429798 [100%]
CA1-010 - ENSP00000429843 [100%]
CA1-015 - ENSP00000430861 [100%]
CA1-201 - ENSP00000392338 [100%]
CA1-202 - ENSP00000443517 [100%]
CA1-004 - ENSP00000430471 [99%]
CA1-006 - ENSP00000427852 [99%]
CA1-013 - ENSP00000430737 [99%]

Other gene match

ANTIGEN VIEW

CA1-001	CA1-003	CA1-004	CA1-006	CA1-010	CA1-013	CA1-015	CA1-201	CA1-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.