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Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm, nucleoli fibrillar center & the Golgi apparatus.
Antibody dilution
1:93
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA038382
Antibody HPA038389
Antibody HPA061505
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in purkinje cells along with neuronal processes.
Immunohistochemical staining of human colon shows strong cytoplasmic and membrane positivity in glandular cells.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes and strong cytoplasmic and nuclear positivity in bile duct cells.
Expression
RNA: detected in 37 tissues Protein: detected in 42 cell types
RNA: detected in 37 tissues Protein: detected in 56 cell types
RNA: detected in 37 tissues Protein: detected in 48 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:75
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038382
Antibody HPA038389
Antibody HPA061505
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
230.5, 191
230.5, 191
230.5, 191
Antibody dilution
1:250
1:250
1:80
PROTEIN ARRAY
Antibody HPA038382
Antibody HPA038389
Antibody HPA061505
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
