Fields »
ABOUT
HELP
BLOG
ANXA1

ANTIBODY INFORMATION
 
 

Antibody HPA011271

  

Antibody HPA011272

  

Antibody CAB013023

  

Antibody CAB035987

  

Antibody CAB058693

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Zymed
   NCI-CPTC
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA011271   HPA011272   71-3400   CPTC-ANXA1-1   AMAb90558  

Host species

 Rabbit   Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 4   4   4   7   11  

References

 1          

Proper citation

 Atlas Antibodies Cat#HPA011271, RRID:AB_1844883   Atlas Antibodies Cat#HPA011272, RRID:AB_1844884       Atlas Antibodies Cat#AMAb90558, RRID:AB_2665585  

VALIDATION SUMMARY
 
 

Antibody HPA011271

  

Antibody HPA011272

  

Antibody CAB013023

  

Antibody CAB035987

  

Antibody CAB058693

  
Immunocytochemistry


Validated
  

Validated
   Supported   Supported    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  

Validated
  

Validated
  
Western blot

Supported   Supported   Supported   Supported   Supported  
Protein array

Supported   Supported        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA011271

  

Antibody HPA011272

  

Antibody CAB013023

  

Antibody CAB035987

  

Antibody CAB058693

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA011272.		  
Validated

Antibody staining overlaps with antibody HPA011271.		        
 

Standard validation

 Supported   Supported   Supported   Supported    

         

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol.    

Antibody dilution

 1:109   1:14   1:200   1:2500    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA011271

  

Antibody HPA011272

  

Antibody CAB013023

  

Antibody CAB035987

  

Antibody CAB058693

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
   
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		        

 
       

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA011272 across 44 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA011271 across 44 cell lines.		        
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		        

 
       

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA011272 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA011271 across 74 cell types.		        
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human esophagus shows cytoplasmic and nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human esophagus shows cytoplasmic and nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human esophagus shows nuclear and cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human esophagus shows cytoplasmic and nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human esophagus shows cytoplasmic and nuclear positivity in squamous epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 40 cell types		   RNA: detected in 37 tissues
Protein: detected in 41 cell types		   RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 45 cell types		   RNA: detected in 37 tissues
Protein: detected in 49 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1250   1:125   1:1500   1:15000   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA011271

  

Antibody HPA011272

  

Antibody CAB013023

  

Antibody CAB035987

  

Antibody CAB058693

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

         

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 38.7   38.7, 22.8, 12.6   38.7, 22.8, 12.6   38.7, 22.8, 12.6   38.7, 22.8, 12.6  

Antibody dilution

 1:250   1:250   1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA011271

  

Antibody HPA011272

  

Antibody CAB013023

  

Antibody CAB035987

  

Antibody CAB058693

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:3000   1:500        

ANTIGEN INFORMATION
 
 

Antibody HPA011271

  

Antibody HPA011272

  

Antibody CAB013023

  

Antibody CAB035987

  

Antibody CAB058693

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein   Recombinant protein  

Length (aa)

 126   102        

Antigen sequence

 
FRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTIL
TTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKP
AFFAEKLHQAMKGVGTRHKALIRIMV
  
MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAA
LHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKAL
TG
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					ANXA1-002 - ENSP00000257497 [100%] 
ANXA1-003 - ENSP00000366109 [100%] 

											 
																						ANXA1-002 - ENSP00000257497 [100%] 
ANXA1-003 - ENSP00000366109 [100%] 
ANXA1-004 - ENSP00000412489 [100%] 
ANXA1-006 - ENSP00000414013 [100%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ANXA1-002
ANXA1-003
ANXA1-004
ANXA1-006
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA011271

												
										 
																				
											
											
											
Antibody HPA011272

												
										 
																				
											
											
											
Antibody CAB013023

												
										 
																				
											
											
											
Antibody CAB035987

												
										 
																				
											
											
											
Antibody CAB058693

												
										 
																		

																	

										
											
1.

												
																						Antibodies biotinylated using a synthetic Z-domain from protein A provide stringent in situ protein detection

	Andersson S et al

	J Histochem Cytochem 2013;61(11):773-84

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.