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GOLM1

ANTIBODY INFORMATION
 
 

Antibody HPA010638

  

Antibody HPA011929

  

Antibody CAB015341

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA010638   HPA011929   sc-48010  

Host species

 Rabbit   Rabbit   Goat  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 4   4   4  

References

 2      

Proper citation

 Atlas Antibodies Cat#HPA010638, RRID:AB_1849886   Atlas Antibodies Cat#HPA011929, RRID:AB_1849888   Santa Cruz Biotechnology Cat#sc-48010, RRID:AB_2113334  

VALIDATION SUMMARY
 
 

Antibody HPA010638

  

Antibody HPA011929

  

Antibody CAB015341

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA010638

  

Antibody HPA011929

  

Antibody CAB015341

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA011929.		  
Validated

Antibody staining overlaps with antibody HPA010638.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.    

Antibody dilution

 1:32   1:97    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA010638

  

Antibody HPA011929

  

Antibody CAB015341

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

     

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA011929 across 70 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA010638 across 70 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human prostate shows dot like cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 62 cell types		   RNA: detected in 37 tissues
Protein: detected in 37 cell types		   RNA: detected in 37 tissues
Protein: detected in 43 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:200   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA010638

  

Antibody HPA011929

  

Antibody CAB015341

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 45.3   45.3   45.3, 13.7, 9  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA010638

  

Antibody HPA011929

  

Antibody CAB015341

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA010638

  

Antibody HPA011929

  

Antibody CAB015341

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 139   109    

Antigen sequence

 
GKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQ
EPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQ
LVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESE
  
VELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITT
GERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQ
MKEVKEQCE
  

											 
																				

																		

										
											
Matching transcripts

												
																					GOLM1-001 - ENSP00000373364 [100%] 
GOLM1-002 - ENSP00000373363 [100%] 

											 
																						GOLM1-001 - ENSP00000373364 [100%] 
GOLM1-002 - ENSP00000373363 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
GOLM1-001
GOLM1-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA010638

												
										 
																				
											
											
											
Antibody HPA011929

												
										 
																				
											
											
											
Antibody CAB015341

												
										 
																		

																	

										
											
1.

												
																						The tumor-suppressive microRNA-143/145 cluster inhibits cell migration and invasion by targeting GOLM1 in prostate cancer

	Kojima S et al

	J Hum Genet 2014;59(2):78-87

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						The microRNA-23b/27b/24-1 cluster is a disease progression marker and tumor suppressor in prostate cancer

	Goto Y et al

	Oncotarget 2014;5(17):7748-59

			Application: WB				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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