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ANTIBODY INFORMATION
 
 

Antibody HPA002657

  

Antibody HPA077243

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002657   HPA077243  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA002657, RRID:AB_1078398    

VALIDATION SUMMARY
 
 

Antibody HPA002657

  

Antibody HPA077243

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry

Uncertain    
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002657

  

Antibody HPA077243

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.  

Antibody dilution

   1:93  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002657

  

Antibody HPA077243

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human endometrium shows distinct luminal membranous positivity in glandular cells.    

Expression

 RNA: detected in 35 tissues
Protein: detected in 8 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:600    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA002657

  

Antibody HPA077243

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 50.5, 28.3   50.5  

Antibody dilution

 1:250   1:211  

PROTEIN ARRAY
 
 

Antibody HPA002657

  

Antibody HPA077243

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3700  

ANTIGEN INFORMATION
 
 

Antibody HPA002657

  

Antibody HPA077243

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 135   69  

Antigen sequence

 
LKTVAQNYSSVTHLHSIGKSVKGRNLWVLVVGRFPKEHRIGIPEFKYVAN
MHGDETVGRELLLHLIDYLVTSDGKDPEITNLINSTRIHIMPSMNPDGFE
AVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVS
  
LLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQLDS
IPVSNPSCPMIPLYRNLPD
 

Matching transcripts

 CPM-001 - ENSP00000448517 [100%]
CPM-002 - ENSP00000447255 [100%]
CPM-005 - ENSP00000446799 [100%]
CPM-201 - ENSP00000339157 [100%]
  CPM-001 - ENSP00000448517 [100%]
CPM-002 - ENSP00000447255 [100%]
CPM-201 - ENSP00000339157 [100%]
 

Other gene match

    
ANTIGEN VIEW
CPM-001
CPM-002
CPM-005
CPM-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.