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SUCLA2

ANTIBODY INFORMATION
 
 

Antibody HPA039435

  

Antibody HPA039536

  

Antibody HPA061528

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039435   HPA039536   HPA061528  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 7   12   13  

References

      

Proper citation

 Atlas Antibodies Cat#HPA039435, RRID:AB_10673144   Atlas Antibodies Cat#HPA039536, RRID:AB_2676561   Atlas Antibodies Cat#HPA061528, RRID:AB_2684553  

VALIDATION SUMMARY
 
 

Antibody HPA039435

  

Antibody HPA039536

  

Antibody HPA061528

  
Immunocytochemistry

 

Validated
  

Validated
  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039435

  

Antibody HPA039536

  

Antibody HPA061528

  

Formal validation: Independent

  
Validated

Antibody staining overlaps with antibody HPA061528.		  
Validated

Antibody staining overlaps with antibody HPA039536.		  
 

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.   Immunofluorescent staining of human cell line HEK 293 shows localization to mitochondria.  

Antibody dilution

   1:65   1:37  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039435

  

Antibody HPA039536

  

Antibody HPA061528

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:200   1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  
 

Standard validation

   Supported    

     

Figure description

   Neuronal expression (soma) in specific nuclei, synaptic staining widespread.    

WESTERN BLOT
 
 

Antibody HPA039435

  

Antibody HPA039536

  

Antibody HPA061528

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 50.3, 8.6   50.3, 21.1   50.3, 32, 17.1  

Antibody dilution

 1:250   1:250   1:120  

PROTEIN ARRAY
 
 

Antibody HPA039435

  

Antibody HPA039536

  

Antibody HPA061528

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:1350  

ANTIGEN INFORMATION
 
 

Antibody HPA039435

  

Antibody HPA039536

  

Antibody HPA061528

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 85   85   95  

Antigen sequence

 
FGGIMRCDVIAQGIVMAVKDLEIKIPVVVRLQGTRVDDAKALIADSGLKI
LACDDLDEAARMVVKLSEIVTLAKQAHVDVKFQLP
  
ATLRNHRPRTAQRAAAQVLGSSGLFNNHGLQVQQQQQRNLSLHEYMSMEL
LQEAGVSVPKGYVAKSPDEAYAIAKKLGSKDVVIK
  
PIDIEEGIKKEQALQLAQKMGFPPNIVESAAENMVKLYSLFLKYDATMIE
INPMVEDSDGAVLCMDAKINFDSNSAYRQKKIFDLQDWTQEDERD
 

Matching transcripts

 SUCLA2-001 - ENSP00000367923 [100%]
SUCLA2-007 - ENSP00000489055 [93%]
  SUCLA2-001 - ENSP00000367923 [100%]
SUCLA2-004 - ENSP00000488974 [100%]
  SUCLA2-001 - ENSP00000367923 [100%]
SUCLA2-009 - ENSP00000415091 [100%]
SUCLA2-010 - ENSP00000392771 [100%]
 

Other gene match

      
ANTIGEN VIEW
SUCLA2-001
SUCLA2-004
SUCLA2-007
SUCLA2-009
SUCLA2-010
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.