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RAPGEF5

ANTIBODY INFORMATION
 
 

Antibody HPA057821

  

Antibody HPA076772

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA057821   HPA076772  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA057821, RRID:AB_2683538   Atlas Antibodies Cat#HPA076772, RRID:AB_2686814  

VALIDATION SUMMARY
 
 

Antibody HPA057821

  

Antibody HPA076772

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Uncertain    
Western blot

Uncertain    
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA057821

  

Antibody HPA076772

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & nuclear bodies.  

Antibody dilution

   1:32  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA057821

  

Antibody HPA076772

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human spleen shows distinct nuclear positivity in cells of the spleen.    

Expression

 RNA: detected in 36 tissues
Protein: detected in 77 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:300    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA057821

  

Antibody HPA076772

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 84.7, 67.7    

Antibody dilution

 1:90    

PROTEIN ARRAY
 
 

Antibody HPA057821

  

Antibody HPA076772

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000   1:1150  

ANTIGEN INFORMATION
 
 

Antibody HPA057821

  

Antibody HPA076772

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 70   78  

Antigen sequence

 
EEDLALVAITFSGEKHELQPNDLVISKSLEASGRIYVYRKDLADTLNPFA
ENEESQQRSMRILGMNTWDL
  
DFLLTYTVFMTTDDLCQALLRHYSAKKYQGKEENSDVPRRKRKVLHLVSQ
WIALYKDWLPEDEHSKMFLKTIYRNVLD
 

Matching transcripts

 RAPGEF5-001 - ENSP00000384044 [100%]
RAPGEF5-201 - ENSP00000343656 [100%]
  RAPGEF5-001 - ENSP00000384044 [100%]
RAPGEF5-201 - ENSP00000343656 [100%]
RAPGEF5-202 - ENSP00000479340 [100%]
RAPGEF5-008 - ENSP00000415664 [88%]
 

Other gene match

    
ANTIGEN VIEW
RAPGEF5-001
RAPGEF5-008
RAPGEF5-201
RAPGEF5-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.