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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:8
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA045284
Antibody HPA048453
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human liver shows strong cytoplasmic and membranous positivity in hepatocytes.
Expression
RNA: detected in 17 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045284
Antibody HPA048453
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
184.1
184.1
Antibody dilution
1:250
1:80
PROTEIN ARRAY
Antibody HPA045284
Antibody HPA048453
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:650
ANTIGEN INFORMATION
Antibody HPA045284
Antibody HPA048453
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
45
52
Antigen sequence
LGRAHIRAHTPACHLLGEVQDSELEGGLAAISACDGLKGVFLLSN
WDLQTVAVWGTFLPTTLTGLGHTPEPALNPGPKGQPESLSPEVPLSSRLL
SM
