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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Antibody dilution
1:122
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA040454
Standard validation
Approved
Figure description
Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in astrocytes.
Expression
RNA: detected in 10 tissues Protein: detected in 16 cell types
Retrieval
HIER pH6
Antibody dilution
1:3500
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Standard validation
Approved
Figure description
Staining observed in subsets of astrocytes, most abundant in hippocampus and subiculum. End feet positivity was seen as accentuation to blood vessel walls.
WESTERN BLOT
Antibody HPA040454
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
51
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA040454
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
