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ADAM10

ANTIBODY INFORMATION
 
 

Antibody HPA050670

  

Antibody CAB001709

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA050670   AB19026  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 13   1  

References

    

Proper citation

    

VALIDATION SUMMARY
 
 

Antibody HPA050670

  

Antibody CAB001709

  
Immunocytochemistry

Uncertain   Supported  
Immunohistochemistry

  Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA050670

  

Antibody CAB001709

  

Standard validation

 Uncertain   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & vesicles.  

Antibody dilution

 1:29   1:400  

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA050670

  

Antibody CAB001709

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human stomach shows moderate cytoplasmic and membranous positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 35 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:750  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA050670

  

Antibody CAB001709

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 84.1   84.1, 16, 14.5, 13.2, 7.5, 6.4, 4.1  

Antibody dilution

 1:140   1:500  

PROTEIN ARRAY
 
 

Antibody HPA050670

  

Antibody CAB001709

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA050670

  

Antibody CAB001709

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 113    

Antigen sequence

 
KHQRAKRAVSHEDQFLRLDFHAHGRHFNLRMKRDTSLFSDEFKVETSNKV
LDYDTSHIYTGHIYGEEGSFSHGSVIDGRFEGFIQTRGGTFYVEPAERYI
KDRTLPFHSVIYH
  

											 
																				

																		

										
											
Matching transcripts

												
																					ADAM10-001 - ENSP00000260408 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ADAM10-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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