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ITGAV

ANTIBODY INFORMATION
 
 

Antibody HPA004856

  

Antibody CAB002499

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA004856   NCL-CD51  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 4   1  

References

    

Proper citation

 Atlas Antibodies Cat#HPA004856, RRID:AB_1846316    

VALIDATION SUMMARY
 
 

Antibody HPA004856

  

Antibody CAB002499

  
Immunocytochemistry

Supported    
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004856

  

Antibody CAB002499

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.    

Antibody dilution

 1:15    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004856

  

Antibody CAB002499

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.   Immunohistochemical staining of human urinary bladder shows strong membranous positivity in urothelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 47 cell types		  

Retrieval

 HIER pH6   HIER pH9  

Antibody dilution

 1:75   1:200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004856

  

Antibody CAB002499

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 116, 112.3, 111.1, 15.3   116, 112.3, 111.1, 15.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004856

  

Antibody CAB002499

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA004856

  

Antibody CAB002499

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 101    

Antigen sequence

 
DHLITKRDLALSEGDIHTLGCGVAQCLKIVCQVGRLDRGKSAILYVKSLL
WTETFMNKENQNHSYSLKSSASFNVIEFPYKNLPIEDITNSTLVTTNVTW
G
  

											 
																				

																		

										
											
Matching transcripts

												
																					ITGAV-001 - ENSP00000261023 [100%] 
ITGAV-003 - ENSP00000364042 [100%] 
ITGAV-004 - ENSP00000404291 [100%] 
ITGAV-008 - ENSP00000389442 [93%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ITGAV-001
ITGAV-003
ITGAV-004
ITGAV-008
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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