We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:88
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA039975
Antibody HPA040382
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human testis shows moderate positivity in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts while Leydig cells displayed strong cytoplasmic and nuclear staining.
Expression
RNA: detected in 34 tissues Protein: detected in 72 cell types
RNA: detected in 34 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:750
1:600
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039975
Antibody HPA040382
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413117)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413117)
Target mass (kDa)
60.6
60.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA039975
Antibody HPA040382
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
