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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol.
Antibody dilution
1:9
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA002087
Antibody CAB019405
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
Immunohistochemical staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
Expression
RNA: detected in 30 tissues Protein: detected in 15 cell types
RNA: detected in 30 tissues Protein: detected in 45 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:125
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002087
Antibody CAB019405
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402529)
Target mass (kDa)
44.2
44.2, 42.7, 41.2, 34.1, 18.8, 15.8
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA002087
Antibody CAB019405
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
