KIF21A - Antibodies - The Human Protein Atlas

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KIF21A

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA058432

Antibody CAB022079

Provider

Atlas Antibodies
Sigma-Aldrich

Upstate

Product name

HPA058432

07-529

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA058432, RRID:AB_2683717

VALIDATION SUMMARY

Antibody HPA058432

Antibody CAB022079

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA058432

Antibody CAB022079

Formal validation: Tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Figure description

Immunofluorescent staining of transgenic HeLa cells show antibody staining in plasma membrane and GFP expression in plasma membrane.

Antibody dilution

1:23

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane & cytosol.

Antibody dilution

1:23

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA058432

Antibody CAB022079

Standard validation

Approved

Figure description

Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.

Immunohistochemical staining of human cerebral cortex shows strong positivity in neurons.

Expression

RNA: detected in 36 tissues
Protein: detected in 33 cell types

RNA: detected in 36 tissues
Protein: detected in 71 cell types

Retrieval

HIER pH6

Antibody dilution

1:35

1:225

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA058432

Antibody CAB022079

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

187.2, 185.5, 182.9, 180.7, 108.7, 71.5

187.2, 185.5, 182.9, 180.7, 108.7, 71.5, 32.5, 15.3

Antibody dilution

1:80

1:500

PROTEIN ARRAY

Antibody HPA058432

Antibody CAB022079

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:600

ANTIGEN INFORMATION

Antibody HPA058432

Antibody CAB022079

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

QKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQ
DLDSVPLENVEDSTDEDAPLNS

Matching transcripts

KIF21A-001 - ENSP00000354851 [100%]
KIF21A-003 - ENSP00000354878 [100%]
KIF21A-004 - ENSP00000447765 [100%]
KIF21A-008 - ENSP00000438075 [100%]
KIF21A-002 - ENSP00000445606 [90%]
KIF21A-005 - ENSP00000448792 [90%]

Other gene match

ANTIGEN VIEW

KIF21A-001	KIF21A-002	KIF21A-003	KIF21A-004	KIF21A-005	KIF21A-008

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.