DPEP3 - Antibodies - The Human Protein Atlas

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DPEP3

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA031870

Antibody HPA058607

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA031870

HPA058607

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA031870, RRID:AB_2674079

Atlas Antibodies Cat#HPA058607, RRID:AB_2683775

VALIDATION SUMMARY

Antibody HPA031870

Antibody HPA058607

Immunohistochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA031870

Antibody HPA058607

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA058607 across 72 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA031870 across 72 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.

Immunohistochemical staining of human testis shows strong cytoplasmic and membranous positivity in cells in seminiferus ducts.

Expression

RNA: detected in 2 tissues
Protein: detected in 2 cell types

RNA: detected in 2 tissues
Protein: detected in 1 cell types

Retrieval

HIER pH6

Antibody dilution

1:8

1:75

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA031870

Antibody HPA058607

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

56.4

Antibody dilution

1:250

1:140

PROTEIN ARRAY

Antibody HPA031870

Antibody HPA058607

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:1650

ANTIGEN INFORMATION

Antibody HPA031870

Antibody HPA058607

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

PRALSTLGSPSLFTTPGVPSALTTPGLTTPGTPKTLDLRGRAQALMRSFP
L

KVREESRAQSPVEAEFPYGQLSTSCHSHLVPQNGHQATHLEVTKQPTNRV

Matching transcripts

DPEP3-001 - ENSP00000268793 [100%]

Other gene match

ANTIGEN VIEW

DPEP3-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.