Fields »
ABOUT
HELP
BLOG
SCYL1

ANTIBODY INFORMATION
 
 

Antibody HPA015015

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA015015  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

  

Proper citation

 Atlas Antibodies Cat#HPA015015, RRID:AB_1856643  

VALIDATION SUMMARY
 
 

Antibody HPA015015

  
Immunocytochemistry

Supported  
Immunohistochemistry

Uncertain  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015015

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

 1:109  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015015

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells and endothelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 17 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:200  

Literature conformity

 Not consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015015

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 89.6, 88.1, 86.4, 86.2, 85.7, 73.7  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA015015

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA015015

  

Antigen

 Recombinant protein fragment  

Length (aa)

 128  

Antigen sequence

 
AFPEDFCRHKVLPQLLTAFEFGNAGAVVLTPLFKVGKFLSAEEYQQKIIP
VVVKMFSSTDRAMRIRLLQQMEQFIQYLDEPTVNTQIFPHVVHGFLDTNP
AIREQTVKSMLLLAPKLNEANLNVELMK
 

Matching transcripts

 SCYL1-001 - ENSP00000270176 [100%]
SCYL1-002 - ENSP00000408192 [100%]
SCYL1-003 - ENSP00000436993 [100%]
SCYL1-005 - ENSP00000432175 [100%]
SCYL1-006 - ENSP00000437254 [100%]
SCYL1-014 - ENSP00000431635 [100%]
 

Other gene match

  
ANTIGEN VIEW
SCYL1-001
SCYL1-002
SCYL1-003
SCYL1-005
SCYL1-006
SCYL1-014
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.